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KMID : 0359319930330010081
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Korean Journal of Veterinary Research
1993 Volume.33 No. 1 p.81 ~ p.85
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Separation of large DNA molecules by pulsed field gel electrophoresis
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Thomas A. Casey
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Abstract
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Gel electrophoresis has proven to be one of the most useful of DNA separation and purification. The new technique of pulsed field gel electrophoresis (PFGE) is high resolution separation of large size DNA moleculs. Conventional continuous gel electrophresis can not be separation of large DNA fragments(20∼50 k base). Field inversion gel electrophoresis (FIGE) is very useful for large DNA molecules. We have found that a pulse ratio ; 2 : 1, time : 24hrs., volts ; 10volts/㎝, start : 0.45sec, end : 1sec, is most effectively resolves DNA fragment in the 6∼50k base.
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